expression plasmid vector for he4  (OriGene)

Journal: International Journal of Oncology

Article Title: High levels of human epididymis protein 4 mRNA and protein expression are associated with chemoresistance and a poor prognosis in pancreatic cancer

doi: 10.3892/ijo.2020.5147

Figure Lengend Snippet: Identification of chemotherapy resistance molecules. (A and B) Results of NGS analysis of (A) GEM and (B) GEM + nab-PTX administration. The genes are shown on the horizontal axis, and the treatment resistance score is shown on the vertical axis. Treatment resistance score was calculated by the ratio of NE value (treated group/control group) multiplied by the difference in NE value (treated group-control group). In both cases (A and B), HE4 was upregulated in the treatment group. (C and D) The NE value of HE4 mRNA. The NE value ratio of HE4 expression between the treatment and control groups was often >1.0 in PDXs treated with GEM or GEM plus nab-PTX. HE4, human epididymis protein 4; PDX, patient-derived xenograft; GEM, gemcitabine; nab-PTX, nab-paclitaxel.

Article Snippet: For transient overexpression experiments, an expression plasmid vector for HE4 (pCMV6-AC-HE4-GFP tag plasmid, NM_006418) was obtained from OriGene Technologies, Inc. and an empty vector (pCMV6-AC-GFP) was adopted for control transfection.

Techniques: Expressing, Derivative Assay

Journal: International Journal of Oncology

Article Title: High levels of human epididymis protein 4 mRNA and protein expression are associated with chemoresistance and a poor prognosis in pancreatic cancer

doi: 10.3892/ijo.2020.5147

Figure Lengend Snippet: Kaplan-Meier plots demonstrating the correlation between HE4 mRNA expression and patient survival in TCGA database for pancreatic cancer (n=166). Red line, high expression (n=34); blue line, low expression (n=132). HE4, human epididymis protein 4. The asterisk (*) indicates a statistically significant difference (P<0.05).

Article Snippet: For transient overexpression experiments, an expression plasmid vector for HE4 (pCMV6-AC-HE4-GFP tag plasmid, NM_006418) was obtained from OriGene Technologies, Inc. and an empty vector (pCMV6-AC-GFP) was adopted for control transfection.

Techniques: Expressing

Journal: International Journal of Oncology

Article Title: High levels of human epididymis protein 4 mRNA and protein expression are associated with chemoresistance and a poor prognosis in pancreatic cancer

doi: 10.3892/ijo.2020.5147

Figure Lengend Snippet: Expression of HE4 and cell viability assays of cell lines. (A) The levels of HE4 gene expression in 9 tumor cell lines (SK-N-AS, KATOIII, ASPC-1, PANC-1, SUIT-2, MIA-Paca2, IMR-32, HCT116 and HeLa) and 2 non-cancerous cell lines (HUVECs and HRGECs) measured by RT-qPCR. Endogenous HE4 expression levels differed among cell lines. * P<0.05. (B) Cell viability assays of tumor cell lines in response to GEM treatment. When the concentration of GEM was increased, the relative viable cell numbers tended to be higher in the cell lines in which expression of endogenous HE4 was relatively higher. Cell lines with different lowercase letters (a-g) indicate statistically significant differences at each GEM concentration. a P<0.05 vs. b-g; b P<0.05 vs. c-g; c P<0.05 vs. d-g; d P<0.05 vs. e-g; e P<0.05 vs. f and g; and f P<0.05 vs. g. HE4, human epididymis protein 4; GEM, gemcitabine; HUVECs, human umbilical vein endothelial cells; HRGECs, human renal glomerular endothelial cells.

Article Snippet: For transient overexpression experiments, an expression plasmid vector for HE4 (pCMV6-AC-HE4-GFP tag plasmid, NM_006418) was obtained from OriGene Technologies, Inc. and an empty vector (pCMV6-AC-GFP) was adopted for control transfection.

Techniques: Expressing, Quantitative RT-PCR, Concentration Assay

Journal: International Journal of Oncology

Article Title: High levels of human epididymis protein 4 mRNA and protein expression are associated with chemoresistance and a poor prognosis in pancreatic cancer

doi: 10.3892/ijo.2020.5147

Figure Lengend Snippet: Viability assay of 293 cells. (A) Expression of HE4 and control vectors in 293 cells followed by treatment with GEM after 24 h (day 1). MTT assay was performed 48 h following treatment with GEM at various concentrations (day 3). (B and C) Histograms representing the rates of change of each measured OD value of control vector and HE4-expressing 293 cells. * P<0.0007, control vector vs. HE4-expressing cells at each GEM concentration, as determined by two-way ANOVA for multiple comparisons with the Bonferroni post hoc test. HE4, human epididymis protein 4; GEM, gemcitabine.

Article Snippet: For transient overexpression experiments, an expression plasmid vector for HE4 (pCMV6-AC-HE4-GFP tag plasmid, NM_006418) was obtained from OriGene Technologies, Inc. and an empty vector (pCMV6-AC-GFP) was adopted for control transfection.

Techniques: Viability Assay, Expressing, MTT Assay, Plasmid Preparation, Concentration Assay

Journal: International Journal of Oncology

Article Title: High levels of human epididymis protein 4 mRNA and protein expression are associated with chemoresistance and a poor prognosis in pancreatic cancer

doi: 10.3892/ijo.2020.5147

Figure Lengend Snippet: Association between HE4 expression and patient prognosis. (A) IHC analysis of HE4 in patient tissues (magnification, ×100). Images are of the same sample tissue sections and representing the intensity of stain. Images in the left panel show H&E staining, and those in the right indicate IHC detection of HE4. (B) The criteria for evaluation of HE4 expression levels. The intensity of stain and percentage criteria are designated. (C) Kaplan-Meier survival analysis in pancreatic cancer patients (n=80). Overall survival is shown in accordance with the protein expression level of HE4. The red line represents the group of patients with high HE4 expression (n=44), and the blue line indicates those with low HE4 expression (n=36). HE4, human epididymis protein 4.

Article Snippet: For transient overexpression experiments, an expression plasmid vector for HE4 (pCMV6-AC-HE4-GFP tag plasmid, NM_006418) was obtained from OriGene Technologies, Inc. and an empty vector (pCMV6-AC-GFP) was adopted for control transfection.

Techniques: Expressing, Staining

Journal: International Journal of Oncology

Article Title: High levels of human epididymis protein 4 mRNA and protein expression are associated with chemoresistance and a poor prognosis in pancreatic cancer

doi: 10.3892/ijo.2020.5147

Figure Lengend Snippet: Association between HE4 expression and clinicopathological features in 80 cases of pancreatic cancer.

Article Snippet: For transient overexpression experiments, an expression plasmid vector for HE4 (pCMV6-AC-HE4-GFP tag plasmid, NM_006418) was obtained from OriGene Technologies, Inc. and an empty vector (pCMV6-AC-GFP) was adopted for control transfection.

Techniques: Expressing

Journal: International Journal of Oncology

Article Title: High levels of human epididymis protein 4 mRNA and protein expression are associated with chemoresistance and a poor prognosis in pancreatic cancer

doi: 10.3892/ijo.2020.5147

Figure Lengend Snippet: Univariate and multivariate analyses of prognostic factor for overall survival in 80 pancreatic cancer patients.

Article Snippet: For transient overexpression experiments, an expression plasmid vector for HE4 (pCMV6-AC-HE4-GFP tag plasmid, NM_006418) was obtained from OriGene Technologies, Inc. and an empty vector (pCMV6-AC-GFP) was adopted for control transfection.

Techniques: